cfx96 real time system c1000 touchthermal cycler (Bio-Rad)
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cfx96 real time system c1000 touchthermal cycler
Cfx96 Real Time System C1000 Touchthermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 27901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1000+touchthermal+cycler/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pm41338226-102-7-13
Average 96 stars, based on 27901 article reviews
Cfx96 Real Time System C1000 Touchthermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 27901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c1000+touchthermal+cycler/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pm41338226-102-7-13
Average 96 stars, based on 27901 article reviews
cfx96 real time system c1000 touchthermal cycler - by Bioz Stars,
2026-09
96/100 stars
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Generated:Article Title: Twist1-induced suppression of oncogene-induced senescence in non-small cell lung cancer requires the transactivation domain of Twist1. Article Snippet: RTPCR primers: RT-PCR primers (IDT-DNA): Reference gene human GAPDH Forward [5′- ACATCGCTCAGACACCATG-3′]; Reference gene human GAPDH Reverse [5′-TGTAGTTGAGGTCAATGAAGGG-3′]; human CDKN2A/p16 Forward [5′-CCAACGCACCGAATAGTTACG-3′]; human CDKN2A/p16 Reverse [5′-CGCTACCTGATTCCAATTCCC-3′]; human CDKN1A/p21 Forward [5′-GCAGACCAGCATGACAGAT-3′]; human CDKN1A/p21 Reverse [5′- GAGACTAAGGCAGAAGATGTAGAG-3′]; human TP53/p53 Forward [5′- GACACGCTTCCCTGGATTG-3′]; human TP53/p53 Reverse [5′-GACGCTAGGATCTGACTGC-3′]; human CDKN1B/p27 Forward [5′-CAGA CGCCCAAGAAGCC-3′]; human CDKN1B/p27 Reverse [5′-AAATTTTCATGTATATCTTCCTTGCTTC-3′]; human CDKN2B/p15 Forward [5′- ACTAGTGGAGAAGGTGCGA-3′]; human CDKN2B/p15 Reverse [5′- GGCGCTGCCCATCATCA-3′]; human OGT Forward [5′-ATACGTTC TTGCTGGTAGTGG-3′]; human OGT Reverse [5′-TGCCTGATATTCTCG ATGTGC-3′]; human MGEA5/OGA Forward [5′-AACTACGTTGCTATCCACACC-3′]; human MGEA5/OGA Reverse [5′-GTCCCATCAACTACACTTGCT-3′]; human GFPT2 Forward [5′-CCATCCAGACCTTGCAGAT3′]; human GFPT2 Reverse [AAATTCACCCGACCTCTCATAG-3′]; human UAP1 Forward [5′-GGCATTTGGAGCATTCATGT-3′]; human UAP1 Reverse [5′-CTGGTTCTGTAGGGTTCGTTT-3′]; human TWIST1 Forward [5′-ATGTCCGCGTCCCACTA-3′]; human TWIST1 Reverse [5′-ACTGTCCATTTTCTCCTTCTCTG-3′]; human Vimentin Forward [5′-CAAGACC TGCTCAATGTTAAGATG-3′]; human Vimentin Reverse [5′-GTGAATCCAGATTAGTTTCCCTCA-3′]. .. Qiagen (RRID:SCR_008539) MYC Targets panel: cDNA samples were generated from 0.4ug total RNA using the RT2 First Strand Kit (#330401, Qiagen). cDNAs were amplified with RT2 SYBR Green qPCR Mastermix (#330501, Qiagen) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) with the Article Title: Twist1-induced suppression of oncogene-induced senescence in non-small cell lung cancer requires the transactivation domain of Twist1 Article Snippet: RTPCR primers: RT-PCR primers (IDT-DNA): Reference gene human GAPDH Forward [5′-ACATCGCTCAGACACCATG-3′]; Reference gene human GAPDH Reverse [5′-TGTAGTTGAGGTCAATGAAGGG-3′]; human CDKN2A/p16 Forward [5′-CCAACGCACCGAATAGTTACG-3′]; human CDKN2A/p16 Reverse [5′-CGCTACCTGATTCCAATTCCC-3′]; human CDKN1A/p21 Forward [5′-GCAGACCAGCATGACAGAT-3′]; human CDKN1A/p21 Reverse [5′-GAGACTAAGGCAGAAGATGTAGAG-3′]; human TP53/p53 Forward [5′-GACACGCTTCCCTGGATTG-3′]; human TP53/p53 Reverse [5′-GACGCTAGGATCTGACTGC-3′]; human CDKN1B/p27 Forward [5′-CAGACGCCCAAGAAGCC-3′]; human CDKN1B/p27 Reverse [5′-AAATTTTCATGTATATCTTCCTTGCTTC-3′]; human CDKN2B/p15 Forward [5′-ACTAGTGGAGAAGGTGCGA-3′]; human CDKN2B/p15 Reverse [5′-GGCGCTGCCCATCATCA-3′]; human OGT Forward [5′-ATACGTTCTTGCTGGTAGTGG-3′]; human OGT Reverse [5′-TGCCTGATATTCTCGATGTGC-3′]; human MGEA5/OGA Forward [5′-AACTACGTTGCTATCCACACC-3′]; human MGEA5/OGA Reverse [5′-GTCCCATCAACTACACTTGCT-3′]; human GFPT2 Forward [5′-CCATCCAGACCTTGCAGAT-3′]; human GFPT2 Reverse [AAATTCACCCGACCTCTCATAG-3′]; human UAP1 Forward [5′-GGCATTTGGAGCATTCATGT-3′]; human UAP1 Reverse [5′-CTGGTTCTGTAGGGTTCGTTT-3′]; human TWIST1 Forward [5′-ATGTCCGCGTCCCACTA-3′]; human TWIST1 Reverse [5′-ACTGTCCATTTTCTCCTTCTCTG-3′]; human Vimentin Forward [5′-CAAGACCTGCTCAATGTTAAGATG-3′]; human Vimentin Reverse [5′-GTGAATCCAGATTAGTTTCCCTCA-3′]. .. Qiagen (RRID:SCR_008539) MYC Targets panel: cDNA samples were generated from 0.4ug total RNA using the RT2 First Strand Kit (#330401, Qiagen). cDNAs were amplified with RT2 SYBR Green qPCR Mastermix (#330501, Qiagen) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) with the Amplification:Article Title: Twist1-induced suppression of oncogene-induced senescence in non-small cell lung cancer requires the transactivation domain of Twist1. Article Snippet: RTPCR primers: RT-PCR primers (IDT-DNA): Reference gene human GAPDH Forward [5′- ACATCGCTCAGACACCATG-3′]; Reference gene human GAPDH Reverse [5′-TGTAGTTGAGGTCAATGAAGGG-3′]; human CDKN2A/p16 Forward [5′-CCAACGCACCGAATAGTTACG-3′]; human CDKN2A/p16 Reverse [5′-CGCTACCTGATTCCAATTCCC-3′]; human CDKN1A/p21 Forward [5′-GCAGACCAGCATGACAGAT-3′]; human CDKN1A/p21 Reverse [5′- GAGACTAAGGCAGAAGATGTAGAG-3′]; human TP53/p53 Forward [5′- GACACGCTTCCCTGGATTG-3′]; human TP53/p53 Reverse [5′-GACGCTAGGATCTGACTGC-3′]; human CDKN1B/p27 Forward [5′-CAGA CGCCCAAGAAGCC-3′]; human CDKN1B/p27 Reverse [5′-AAATTTTCATGTATATCTTCCTTGCTTC-3′]; human CDKN2B/p15 Forward [5′- ACTAGTGGAGAAGGTGCGA-3′]; human CDKN2B/p15 Reverse [5′- GGCGCTGCCCATCATCA-3′]; human OGT Forward [5′-ATACGTTC TTGCTGGTAGTGG-3′]; human OGT Reverse [5′-TGCCTGATATTCTCG ATGTGC-3′]; human MGEA5/OGA Forward [5′-AACTACGTTGCTATCCACACC-3′]; human MGEA5/OGA Reverse [5′-GTCCCATCAACTACACTTGCT-3′]; human GFPT2 Forward [5′-CCATCCAGACCTTGCAGAT3′]; human GFPT2 Reverse [AAATTCACCCGACCTCTCATAG-3′]; human UAP1 Forward [5′-GGCATTTGGAGCATTCATGT-3′]; human UAP1 Reverse [5′-CTGGTTCTGTAGGGTTCGTTT-3′]; human TWIST1 Forward [5′-ATGTCCGCGTCCCACTA-3′]; human TWIST1 Reverse [5′-ACTGTCCATTTTCTCCTTCTCTG-3′]; human Vimentin Forward [5′-CAAGACC TGCTCAATGTTAAGATG-3′]; human Vimentin Reverse [5′-GTGAATCCAGATTAGTTTCCCTCA-3′]. .. Qiagen (RRID:SCR_008539) MYC Targets panel: cDNA samples were generated from 0.4ug total RNA using the RT2 First Strand Kit (#330401, Qiagen). cDNAs were amplified with RT2 SYBR Green qPCR Mastermix (#330501, Qiagen) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) with the Article Title: Twist1-induced suppression of oncogene-induced senescence in non-small cell lung cancer requires the transactivation domain of Twist1 Article Snippet: SYBR-green quantitative RT-PCR : Total RNA was isolated using TRIzolTM RNA Isolation Reagent (#15596018, ThermoFisher Sci.) protocol and cleaned with RNeasy mini kit (#74104, Qiagen) according to the manufacturer’s protocol. cDNA was generated from 1ug total RNA using the iScriptTM cDNA synthesis kit (#1708891, Bio-Rad Laboratories). .. Primers were purchased from IDT-DNA. cDNA was amplified with iTaqTM Universal SYBR® Green Supermix (#1725122, Bio-Rad Laboratories) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) with the Article Title: Twist1-induced suppression of oncogene-induced senescence in non-small cell lung cancer requires the transactivation domain of Twist1 Article Snippet: RTPCR primers: RT-PCR primers (IDT-DNA): Reference gene human GAPDH Forward [5′-ACATCGCTCAGACACCATG-3′]; Reference gene human GAPDH Reverse [5′-TGTAGTTGAGGTCAATGAAGGG-3′]; human CDKN2A/p16 Forward [5′-CCAACGCACCGAATAGTTACG-3′]; human CDKN2A/p16 Reverse [5′-CGCTACCTGATTCCAATTCCC-3′]; human CDKN1A/p21 Forward [5′-GCAGACCAGCATGACAGAT-3′]; human CDKN1A/p21 Reverse [5′-GAGACTAAGGCAGAAGATGTAGAG-3′]; human TP53/p53 Forward [5′-GACACGCTTCCCTGGATTG-3′]; human TP53/p53 Reverse [5′-GACGCTAGGATCTGACTGC-3′]; human CDKN1B/p27 Forward [5′-CAGACGCCCAAGAAGCC-3′]; human CDKN1B/p27 Reverse [5′-AAATTTTCATGTATATCTTCCTTGCTTC-3′]; human CDKN2B/p15 Forward [5′-ACTAGTGGAGAAGGTGCGA-3′]; human CDKN2B/p15 Reverse [5′-GGCGCTGCCCATCATCA-3′]; human OGT Forward [5′-ATACGTTCTTGCTGGTAGTGG-3′]; human OGT Reverse [5′-TGCCTGATATTCTCGATGTGC-3′]; human MGEA5/OGA Forward [5′-AACTACGTTGCTATCCACACC-3′]; human MGEA5/OGA Reverse [5′-GTCCCATCAACTACACTTGCT-3′]; human GFPT2 Forward [5′-CCATCCAGACCTTGCAGAT-3′]; human GFPT2 Reverse [AAATTCACCCGACCTCTCATAG-3′]; human UAP1 Forward [5′-GGCATTTGGAGCATTCATGT-3′]; human UAP1 Reverse [5′-CTGGTTCTGTAGGGTTCGTTT-3′]; human TWIST1 Forward [5′-ATGTCCGCGTCCCACTA-3′]; human TWIST1 Reverse [5′-ACTGTCCATTTTCTCCTTCTCTG-3′]; human Vimentin Forward [5′-CAAGACCTGCTCAATGTTAAGATG-3′]; human Vimentin Reverse [5′-GTGAATCCAGATTAGTTTCCCTCA-3′]. .. Qiagen (RRID:SCR_008539) MYC Targets panel: cDNA samples were generated from 0.4ug total RNA using the RT2 First Strand Kit (#330401, Qiagen). cDNAs were amplified with RT2 SYBR Green qPCR Mastermix (#330501, Qiagen) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) with the SYBR Green Assay:Article Title: Twist1-induced suppression of oncogene-induced senescence in non-small cell lung cancer requires the transactivation domain of Twist1. Article Snippet: RTPCR primers: RT-PCR primers (IDT-DNA): Reference gene human GAPDH Forward [5′- ACATCGCTCAGACACCATG-3′]; Reference gene human GAPDH Reverse [5′-TGTAGTTGAGGTCAATGAAGGG-3′]; human CDKN2A/p16 Forward [5′-CCAACGCACCGAATAGTTACG-3′]; human CDKN2A/p16 Reverse [5′-CGCTACCTGATTCCAATTCCC-3′]; human CDKN1A/p21 Forward [5′-GCAGACCAGCATGACAGAT-3′]; human CDKN1A/p21 Reverse [5′- GAGACTAAGGCAGAAGATGTAGAG-3′]; human TP53/p53 Forward [5′- GACACGCTTCCCTGGATTG-3′]; human TP53/p53 Reverse [5′-GACGCTAGGATCTGACTGC-3′]; human CDKN1B/p27 Forward [5′-CAGA CGCCCAAGAAGCC-3′]; human CDKN1B/p27 Reverse [5′-AAATTTTCATGTATATCTTCCTTGCTTC-3′]; human CDKN2B/p15 Forward [5′- ACTAGTGGAGAAGGTGCGA-3′]; human CDKN2B/p15 Reverse [5′- GGCGCTGCCCATCATCA-3′]; human OGT Forward [5′-ATACGTTC TTGCTGGTAGTGG-3′]; human OGT Reverse [5′-TGCCTGATATTCTCG ATGTGC-3′]; human MGEA5/OGA Forward [5′-AACTACGTTGCTATCCACACC-3′]; human MGEA5/OGA Reverse [5′-GTCCCATCAACTACACTTGCT-3′]; human GFPT2 Forward [5′-CCATCCAGACCTTGCAGAT3′]; human GFPT2 Reverse [AAATTCACCCGACCTCTCATAG-3′]; human UAP1 Forward [5′-GGCATTTGGAGCATTCATGT-3′]; human UAP1 Reverse [5′-CTGGTTCTGTAGGGTTCGTTT-3′]; human TWIST1 Forward [5′-ATGTCCGCGTCCCACTA-3′]; human TWIST1 Reverse [5′-ACTGTCCATTTTCTCCTTCTCTG-3′]; human Vimentin Forward [5′-CAAGACC TGCTCAATGTTAAGATG-3′]; human Vimentin Reverse [5′-GTGAATCCAGATTAGTTTCCCTCA-3′]. .. Qiagen (RRID:SCR_008539) MYC Targets panel: cDNA samples were generated from 0.4ug total RNA using the RT2 First Strand Kit (#330401, Qiagen). cDNAs were amplified with RT2 SYBR Green qPCR Mastermix (#330501, Qiagen) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) with the Article Title: Twist1-induced suppression of oncogene-induced senescence in non-small cell lung cancer requires the transactivation domain of Twist1 Article Snippet: SYBR-green quantitative RT-PCR : Total RNA was isolated using TRIzolTM RNA Isolation Reagent (#15596018, ThermoFisher Sci.) protocol and cleaned with RNeasy mini kit (#74104, Qiagen) according to the manufacturer’s protocol. cDNA was generated from 1ug total RNA using the iScriptTM cDNA synthesis kit (#1708891, Bio-Rad Laboratories). .. Primers were purchased from IDT-DNA. cDNA was amplified with iTaqTM Universal SYBR® Green Supermix (#1725122, Bio-Rad Laboratories) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) with the Article Title: Twist1-induced suppression of oncogene-induced senescence in non-small cell lung cancer requires the transactivation domain of Twist1 Article Snippet: RTPCR primers: RT-PCR primers (IDT-DNA): Reference gene human GAPDH Forward [5′-ACATCGCTCAGACACCATG-3′]; Reference gene human GAPDH Reverse [5′-TGTAGTTGAGGTCAATGAAGGG-3′]; human CDKN2A/p16 Forward [5′-CCAACGCACCGAATAGTTACG-3′]; human CDKN2A/p16 Reverse [5′-CGCTACCTGATTCCAATTCCC-3′]; human CDKN1A/p21 Forward [5′-GCAGACCAGCATGACAGAT-3′]; human CDKN1A/p21 Reverse [5′-GAGACTAAGGCAGAAGATGTAGAG-3′]; human TP53/p53 Forward [5′-GACACGCTTCCCTGGATTG-3′]; human TP53/p53 Reverse [5′-GACGCTAGGATCTGACTGC-3′]; human CDKN1B/p27 Forward [5′-CAGACGCCCAAGAAGCC-3′]; human CDKN1B/p27 Reverse [5′-AAATTTTCATGTATATCTTCCTTGCTTC-3′]; human CDKN2B/p15 Forward [5′-ACTAGTGGAGAAGGTGCGA-3′]; human CDKN2B/p15 Reverse [5′-GGCGCTGCCCATCATCA-3′]; human OGT Forward [5′-ATACGTTCTTGCTGGTAGTGG-3′]; human OGT Reverse [5′-TGCCTGATATTCTCGATGTGC-3′]; human MGEA5/OGA Forward [5′-AACTACGTTGCTATCCACACC-3′]; human MGEA5/OGA Reverse [5′-GTCCCATCAACTACACTTGCT-3′]; human GFPT2 Forward [5′-CCATCCAGACCTTGCAGAT-3′]; human GFPT2 Reverse [AAATTCACCCGACCTCTCATAG-3′]; human UAP1 Forward [5′-GGCATTTGGAGCATTCATGT-3′]; human UAP1 Reverse [5′-CTGGTTCTGTAGGGTTCGTTT-3′]; human TWIST1 Forward [5′-ATGTCCGCGTCCCACTA-3′]; human TWIST1 Reverse [5′-ACTGTCCATTTTCTCCTTCTCTG-3′]; human Vimentin Forward [5′-CAAGACCTGCTCAATGTTAAGATG-3′]; human Vimentin Reverse [5′-GTGAATCCAGATTAGTTTCCCTCA-3′]. .. Qiagen (RRID:SCR_008539) MYC Targets panel: cDNA samples were generated from 0.4ug total RNA using the RT2 First Strand Kit (#330401, Qiagen). cDNAs were amplified with RT2 SYBR Green qPCR Mastermix (#330501, Qiagen) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) with the Real-time Polymerase Chain Reaction:Article Title: Twist1-induced suppression of oncogene-induced senescence in non-small cell lung cancer requires the transactivation domain of Twist1. Article Snippet: RTPCR primers: RT-PCR primers (IDT-DNA): Reference gene human GAPDH Forward [5′- ACATCGCTCAGACACCATG-3′]; Reference gene human GAPDH Reverse [5′-TGTAGTTGAGGTCAATGAAGGG-3′]; human CDKN2A/p16 Forward [5′-CCAACGCACCGAATAGTTACG-3′]; human CDKN2A/p16 Reverse [5′-CGCTACCTGATTCCAATTCCC-3′]; human CDKN1A/p21 Forward [5′-GCAGACCAGCATGACAGAT-3′]; human CDKN1A/p21 Reverse [5′- GAGACTAAGGCAGAAGATGTAGAG-3′]; human TP53/p53 Forward [5′- GACACGCTTCCCTGGATTG-3′]; human TP53/p53 Reverse [5′-GACGCTAGGATCTGACTGC-3′]; human CDKN1B/p27 Forward [5′-CAGA CGCCCAAGAAGCC-3′]; human CDKN1B/p27 Reverse [5′-AAATTTTCATGTATATCTTCCTTGCTTC-3′]; human CDKN2B/p15 Forward [5′- ACTAGTGGAGAAGGTGCGA-3′]; human CDKN2B/p15 Reverse [5′- GGCGCTGCCCATCATCA-3′]; human OGT Forward [5′-ATACGTTC TTGCTGGTAGTGG-3′]; human OGT Reverse [5′-TGCCTGATATTCTCG ATGTGC-3′]; human MGEA5/OGA Forward [5′-AACTACGTTGCTATCCACACC-3′]; human MGEA5/OGA Reverse [5′-GTCCCATCAACTACACTTGCT-3′]; human GFPT2 Forward [5′-CCATCCAGACCTTGCAGAT3′]; human GFPT2 Reverse [AAATTCACCCGACCTCTCATAG-3′]; human UAP1 Forward [5′-GGCATTTGGAGCATTCATGT-3′]; human UAP1 Reverse [5′-CTGGTTCTGTAGGGTTCGTTT-3′]; human TWIST1 Forward [5′-ATGTCCGCGTCCCACTA-3′]; human TWIST1 Reverse [5′-ACTGTCCATTTTCTCCTTCTCTG-3′]; human Vimentin Forward [5′-CAAGACC TGCTCAATGTTAAGATG-3′]; human Vimentin Reverse [5′-GTGAATCCAGATTAGTTTCCCTCA-3′]. .. Qiagen (RRID:SCR_008539) MYC Targets panel: cDNA samples were generated from 0.4ug total RNA using the RT2 First Strand Kit (#330401, Qiagen). cDNAs were amplified with RT2 SYBR Green qPCR Mastermix (#330501, Qiagen) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) with the Article Title: Twist1-induced suppression of oncogene-induced senescence in non-small cell lung cancer requires the transactivation domain of Twist1 Article Snippet: SYBR-green quantitative RT-PCR : Total RNA was isolated using TRIzolTM RNA Isolation Reagent (#15596018, ThermoFisher Sci.) protocol and cleaned with RNeasy mini kit (#74104, Qiagen) according to the manufacturer’s protocol. cDNA was generated from 1ug total RNA using the iScriptTM cDNA synthesis kit (#1708891, Bio-Rad Laboratories). .. Primers were purchased from IDT-DNA. cDNA was amplified with iTaqTM Universal SYBR® Green Supermix (#1725122, Bio-Rad Laboratories) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) with the Article Title: Twist1-induced suppression of oncogene-induced senescence in non-small cell lung cancer requires the transactivation domain of Twist1 Article Snippet: RTPCR primers: RT-PCR primers (IDT-DNA): Reference gene human GAPDH Forward [5′-ACATCGCTCAGACACCATG-3′]; Reference gene human GAPDH Reverse [5′-TGTAGTTGAGGTCAATGAAGGG-3′]; human CDKN2A/p16 Forward [5′-CCAACGCACCGAATAGTTACG-3′]; human CDKN2A/p16 Reverse [5′-CGCTACCTGATTCCAATTCCC-3′]; human CDKN1A/p21 Forward [5′-GCAGACCAGCATGACAGAT-3′]; human CDKN1A/p21 Reverse [5′-GAGACTAAGGCAGAAGATGTAGAG-3′]; human TP53/p53 Forward [5′-GACACGCTTCCCTGGATTG-3′]; human TP53/p53 Reverse [5′-GACGCTAGGATCTGACTGC-3′]; human CDKN1B/p27 Forward [5′-CAGACGCCCAAGAAGCC-3′]; human CDKN1B/p27 Reverse [5′-AAATTTTCATGTATATCTTCCTTGCTTC-3′]; human CDKN2B/p15 Forward [5′-ACTAGTGGAGAAGGTGCGA-3′]; human CDKN2B/p15 Reverse [5′-GGCGCTGCCCATCATCA-3′]; human OGT Forward [5′-ATACGTTCTTGCTGGTAGTGG-3′]; human OGT Reverse [5′-TGCCTGATATTCTCGATGTGC-3′]; human MGEA5/OGA Forward [5′-AACTACGTTGCTATCCACACC-3′]; human MGEA5/OGA Reverse [5′-GTCCCATCAACTACACTTGCT-3′]; human GFPT2 Forward [5′-CCATCCAGACCTTGCAGAT-3′]; human GFPT2 Reverse [AAATTCACCCGACCTCTCATAG-3′]; human UAP1 Forward [5′-GGCATTTGGAGCATTCATGT-3′]; human UAP1 Reverse [5′-CTGGTTCTGTAGGGTTCGTTT-3′]; human TWIST1 Forward [5′-ATGTCCGCGTCCCACTA-3′]; human TWIST1 Reverse [5′-ACTGTCCATTTTCTCCTTCTCTG-3′]; human Vimentin Forward [5′-CAAGACCTGCTCAATGTTAAGATG-3′]; human Vimentin Reverse [5′-GTGAATCCAGATTAGTTTCCCTCA-3′]. .. Qiagen (RRID:SCR_008539) MYC Targets panel: cDNA samples were generated from 0.4ug total RNA using the RT2 First Strand Kit (#330401, Qiagen). cDNAs were amplified with RT2 SYBR Green qPCR Mastermix (#330501, Qiagen) using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) with the Polymerase Chain Reaction:Article Title: Assessment of genetically modified soybean MON 94637 (application GMFF‐2023‐21116) Article Snippet: .. The new plate was sealed with a sealing foil with the PX1TM PCR Plate Sealer and run in a Bio Rad Article Title: Assessment of genetically modified soybean DBN9004 (application EFSA‐GMO‐BE‐2019‐165) Article Snippet: .. The new plate was sealed with a sealing foil with the PX1TM PCR Plate Sealer and run in a Bio Rad Article Title: Assessment of genetically modified maize DAS1131 (application GMFF ‐2021‐1530) Article Snippet: .. The new plate was sealed with a sealing foil with the PX1TM PCR Plate Sealer and run in a Bio Rad other:Article Title: Twist1-induced suppression of oncogene-induced senescence in non-small cell lung cancer requires the transactivation domain of Twist1. Article Snippet: SYBR-green quantitative RT-PCR: Total RNA was isolated using TRIzol RNA Isolation Reagent (#15596018, ThermoFisher Sci.) protocol and cleaned with RNeasy mini kit (#74104, Qiagen) according to the manufacturer’s protocol. |